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Sequence 1: part 1
When the cutscene with Mario is over, enable eagle vision and climb up following the highlighted path. Then Follow Mario untill you reach the gates.
Once the gates opened you will be attacked. You must kill all the enemies before you can continue. Keep following Mario across the rooftops until you reach the tower. After the cutscene the game wil really start.
Sequence 1: part 2
Once you reagain control of Ezio, you will be on a horse and you need to follow Mario to the villa.
Once you've reached the villa, there will be three missions available. The first one is just a little to the left from the villa.
For this mission, all you need to do is pick up a box of flowers and follow the woman back to the villa.
The second mission is located right outside the gates at the stables. For this mission you need to catch Mario's escaped horse.
Wait untill the horse ran by you, then run towards it and press the mount button.
When you have mounted the horse, ride it back to the stables and into the white mark.
The final mission is on the castle walls, you can get there using the lift in front of the wall.
After talking to the guards at the cannons, run left on the wall until you have reached the sleeping engineer in the corner.
When the engineer decides to follow you, just walk back to the cannons.
After the engineer fixed the canon, you will be asked to shoot five targets. You can do this by highlighting the targets with your mouse/joystick and pressing the firing button.
After going back to the villa and a very long cutscene, you will find yourself on a galloping horse. You can move to the left or the right to get to the yellow mark on the map.
Once you've reached the mark, Ezio will get off the horse. Climb up the wall and run to the tower in front of you
Use the lift to get on top of the tower and select the hidden blade to air assassinate one or two attackers.
After your air assassination, equip your sword and kill the guards.
When you have killed the guards, run to the cannons and destroy the enemies' (remember you can destroy two or more with one shot).
After yet another cutscene you will need to run towards the villa, but since you can't climb becaus you're wounded and the city is being destroyed there is only one way. You will be attacked by enemies whom you can either kill (reccomended) or just run by them and hope they wont follow you.
If you've reached the villa, you will see Claudia being attacked. Kill her attacker and follow her inside.
Once you're inside, simply run down the stairs and the first sequence will be finished.
Sequence 2: part 1
After a very long cutscene and a self explaining parkour level, you need to find 4 power generators. The first one is located down the stairs at the left side of the villa.
For the second generator, run back up the stairs and rund down the stairs at the right side of the villa.
When you have connected the second generator, climb on the roof directly above it and enable eagle vision again.
Simply jump to the roof right in front of you and the source should be visible.
After connecting the third source, the last one will be on the at the right. You can jump there from the roof you' are on and after connecting that one, another cutscene will start playing.
In the training they will ask you to get some killstreaks on the attackers, however all you need to do is stay alive for 45 seconds to continue playing.
After yet another cutscene, you will be outside a house. walk to the back and walk the stairs all the way to the bottom.
Walk to the right towards the docter. This is mandatory, you cannot heal yourself using a potion.
When the docter healed you, run towards the church and climb it from the front.
When you have reached the top of the tower, move towards the beams and synchronize. When your done simply jump into the hay bales for the next mission to start.
Sequence 2: part 2
Tail the guards by following them (don't come to close but don't lose them out of sight) and blending in with the groups of people.
Keep following the guards until they run by a ladder.
You can either climb on the ladder and continue tailing them over the rooftops or keep blending in with the citizens.
After tailing them long enough they will run towards a citizen and attack him.
Run towards the gards and kill them before they kill the man.
Sequence 2: part 3
Run towards the wall and climb over it.
Climb ontop of the house and move over the horizontal beams until you reach the target.
Equip your hidden blade, highlight the target and press the attack button to air assassinate him (the air assassination is not mandatory but it is needed for 100% synchronization and it's easier).
Equip your sword again as soon as the cutscene is over beacaus you will be attacked by the guards.
To finish the mission either kill the guards or run away and become anonymous.
Sequence 2: part 4
Walk behind Machivelli without being detected by the guards (don't sprint or steal).
Walk up to the shop and buy the item's listed under 'armor' and 'small weapons'.
Now just keep following Machiavelli.
You will find multiple chests and collecaibles along the way, you can get them as long as you don't draw too much attention.
To kill the Borgia master, first kill all the guards surrounding him.
After the guards are killed, go to the Borgia master and grab him. This is not necessary, but it's easier and needed for 100% synchronization.
Walk up to the wooden structure and press the same button you used to grab him to throw him.
Go back to Machiavelli.
Climb up the tower using the ladder.
Ignite the barrels and let the cutscene play.
Now just walk Machiavelli to the stables and the mission will be over.
Sequence 2: part 5
Get on the horse right in front of you and follow Machiavelli.
Make sure you don't knock over any guards and stay close to Machiavelli.
Wait untill the thieve has stolen your money, then start sprinting behind him.
Get in the tackle position (key binding is different from console to console) and run against him.
To start the next mission go talk to Machiavelli once again.
Follow him once again on your horse, still minding the guards and the distance.
Once you've reached the messenger, dismount your horse and kill the guards.
Sprint behind the guard and try to tackle him.
If he climbs on the walls, simply climb behind him and keep following him.
You need to tackle him within the minute for 100% synchronization.
Sequence 2: part 6
After the cutscene you will immediately be attacked. Since you can't take any damage if you want 100 % synchronization, i recommend equiping your sword, waiting for them to attack and then counter attack them.
After you have killed the attackers, walk down the stairs and go trough the little door.
When the cutscene is over, run on the broken pillar and move to the left. When you're inthe possition as shown in the picture, eject back on the other pillar.
From the pillar jump to the wall in front of you, then move to the right and climb on the platform.
From the platform you can jump to the first handle.
When you've activated the first handle, jump back to the platform you came from and sprint towards the other side of the room.
From the second handle, jump to the broken pillar in the middle of the room.
Once you're ontop of it, jump to the one on the right.
Climb ontop of it and jump to the pillar at your left, you normally should grab the ledge.
Slide left on the ledge 'till you can go no further (as shown in the picture), then climb up.
Slide left once again and when you can go no further, eject back.
You will land on a broken wall, from there jump to the platform on the left.
Activate the handle and go back.
Jump on the broken wall and walk to the pointy end closest to the half pillar.
Jump to the pillar, then jum the wall.
Move to the right on the wall until you reach the final handle.
Sequnce 2: part 7
Run trough the big door in front of you. If you go left, you will find a flag (this is a collectable, it's not nescessary to complete the mission.
You need to go trough the door and take a right. then climb the small pillar as shown in the picture.
Jump from the pillar to the wall and move to the right.
Go around the corner and keep moving right.
When you can no longer slide to the right, eject back and keep moving across the ledge until you reach a horizontal wooden beam.
Get on the beam and jump to the white thing hanging from the ceiling.
Run trough the hallway and climb the small pillar.
Jump to the beam and over the gate.
You can use the thing hanging from the ceiling to get over the last gate and open the door via the handle.
Climb on the broken wall in the back of the room.
Jump to the wooden beams that are shown in the picture.
From the beam, jump to the pillar and move to the left until you can back eject to the statue.
From the statue, jump to the broken wall at the opposite side of the room and move to the right.
Keep moving to the right until you can't go further, then eject back onto the other statue.
Run over the hand of the staue and jump towards the wall.
Crawl up the beam and jump ahead to the iron staff coming from the wall.
Run towards the broken pillar and climb it.
Jump to the wall on the left side of the room and move to the right until you reach the little platform.
From the platform, jump to the ledge on the wall and move to the right.
Once you're in the position as shown on the screen, eject back.
Keep moving along the wall until you can eject back and grab the iron grab the iron staff coming from the wall.
After the cutscene, clim on the broken pieces as shown in the picture, and run along towads the wall.
Jump over the wall and run tough the hallway.
Climb on the small pillar at the other side of the hallway and face the iron staff.
Jump to the iron staff and jump from there to the wall.
Move left on the wall until you can go no further and eject back.
Move right across the wall and normally, you should unlock a checkpoint in case you fall.
Climb upwards as in the picture and eject back.
Get up the platform and run up the wall.
Clim to the upper ledge and move to the left. From there eject back and another cutscene will begin.
Climb up the wall next to the skeleton and grab hold of the wooden beam.
Jump from beam to beam until you can jump onto the wall that is shown in the picture.
Run to the end of the wall and jump towards the wooden beam.
Crawl ontop of it and jump to the balustrade on the left.
From the ballustrade, jump to the pilar. This should unlock another checkpoint.
From the pillar, jump to the wall and move to the left.
Once you have reached the end of the ledge, eject back onto the pillar.
Jump from pilar to pilar, another checkpoint should unlock.
Jump from the last pillar onto the wall. climb up and move left to get to the beam.
Crawl op the beam. You can jump left to get the flag, or to the right to finish the mission.
Once you're in the treasure room, you can loot the chests? Only the one on the shrine is necessary for the mission.
After the cutscene, climb on the ladder and press the 'interact' button.
Then simply follow Machiavelli to finish the mission.
For the final mission of this sequence, simply climb the tower and walk to the door.
Sequence 3: part 1
In the beginning you'll have the choice between 3 missions. I started with the one at the bottom of the map.
Before starting this mission i recommend getting either a crossbow or some throwing knives.
Since it's pretty far, call your horse and ride towards the mission.
Walk up to the door and enter to start this mission.
After talking to La Volpe, meet him on the roof that will be marked on your map.
After the cutscene, kill the guards. You can do this from the roof with your crossbow or throwing knives. If you don't have any, jump down and kill them with your sword.
When the guards are killed, run towards the group of people that you see in the picture.
Blend with the group of people and keep following them.
Move towards the people that aren't moving and face the same way as in the picture.
When you see a group of people walking towards the guards in the picture, run towards them and blend in with them.
Keep walking with them until you've reached the guards.
When you passed the guards, run down the stairs and towards the mark.
When you did this you will need to lose your notoriaty.
The easiest way to do this is to kill an eyewitness.
Then climb on the roof and rip the poster from the wall.
When you've lost your notoriaty, go back to your horse and ride to the mark on your map. Go talk to La volpe on the bench.
When you're done talking, just go talk to the architect and the mission will be over.
Sequence 3: part 2
The second mission i did is the one at the brothel.
This is a simple mission, just go to the mark on the map.
You need 2500 florintines. if you don't have that many, you can pickpocket them. Don't worry, you'll get them back.
Make sure you don't fall in the water or you won't get 100% synchronization.
When you've reached the ship and the cutscene has played, pull out your sword and kill the traders. Once again, mind the water.
Since they dodge alot of attacks, the easiest way to kill them is either by counter attacks or by grabbing them (as shown in the picture).
When you've killed them, move back to the brothel and meet the girls on the roof.
To finish this mission, go talk to the architect.
Sequence 3: part 3
Immediately after the cutscene, start sprinting behind the Borgia captain. i recommend locking on him since you don't want to accidentally kill innocent civillians.
When you're close enough, press the attack button to kill him.
When you've killed him, jump in the water towards the island.
Swim towards the bridge shown in the picture.
Climb on the pole and jump to the ledge of the bridge.
Move to the right and climb up the bridge.
Then simply climb the tower and ignite it.
After doing so go to the mark on your map.
Once again, go talk to the architect to finish the mission.
For the final mission all you need to do is go talk with Machiavelli and the others.
When you're done, go talk to the architect one last time to end this sequence.
Sequence 4: part 1
Get on the boat in the river and peddle towards the mark.
Climb the pole shown in the picture.
Jump to the other pole and towards the ledge. Move towards the ledge.
Jump in the haystack and kill the guards that walks by.
Jump out of the stack and get the guards attention, then jump back in.
Kill the first one, the other one will just turn around.
Run towards him and kill him.
Climb the ladder.
After the cutscene, run up the wall and climb it.
Move towards the guard and kill him.
Run towards the haystack and jump in it.
Mind the guard.
Move towards the mark and drop down.
Move to the other side by jumping from obstacle to obstacle. Kill the guard with either a throwing knive or a crossbow.
Jump to the mark and climb up.
Make sure you kill this guard, or he will detect you.
Run over the ropes and towards the mark.Climb the wall, this should be pretty easy and self explanatory.
Once you've reached this point, hang from the beam, drop down and press the 'grab' button repeatedly.
Jump to this beam and climb up the wall.
Once you get to the mark, keep climbing upwards.
Once you're all the way on the top, climb up the roof.
Run towards the first guard and kill him.
Kill the second one with a throwing knive.
Drop down into the mark.
Run down the stairs and towards the mark, mind the guard behind the corner.
Run up the stairs at the end of the hallway.
Run towards the mark.
Kill the guards with your hidden blade, begin with he one in armor (as seen in the picture).
When the guards are dead, run back trough the door you came from and take a right on the stairs.
Climb the wall on your right.
Grab the ledge and move to the left. Drop down once you're over the fence.
Kill the guards that will attack you and run back to the woman.
Take her hostage, either by tackling her or just getting close to her and press the 'grab' button.
Walk down the stairs with her still as a hostage. When she runs away just tackle her and grab her again.
Put her down whenever the guards attack, kill them and grab her again.
Bring her back to the prison of Caterina.
Put Caterina down out of sight and kill the two guards in the hallway.
Grab her and run down the stairs.
Put her down when you see a guard and kill him with your hidden blade.
Take a right and keep going down, i recommend putting Caterina down and killing this guard with a throwing knive.
Kill the other guard with your hidden blade and throw Caterina down.
Kill the guard that is walking towards you by clicking the 'attack' button when he's close enough.
Carry Caterina trough the hallway, watch out for the guard.
Run towards the handles and open them.
Run wth Christina towards the stables and into the mark.
Once you're on your horse, follow Caterina over the bridge. Then just attack the guards until the time runs out and escape the area.
Sequence 4: part 2
Run towards the courtesans and follow the marks.
Keep running towards the directions the courtesans point.
When you're close enough, make sure he is highlighted and press the button to let your recruits kill him.
Go talk to the boy crying on the docks to start the next mission.
Go towards the marker and find your target using eagle vision.
Tail him by blending in with groups of people.
Once he starts sprinting, sprint behind him.
When he stops sprinting, blend in with the group that is seen on the picture.
Run behind him and make sure he is highlighted. Then call your assassin recruits to kill him.
Kill the remaining guards.
When the guards are dead, jump down and run up to the pigeon coop.Once you leave this menu, the mission will be finished.
Sequence 4: part 3
Go hire a group of sellswords.
Run towards the mark on your map.
Use eagle vision to locate the target.
Command your sellswords to kill the overseer.
Walk in the mark at the gate to start the next mission. WARNING: i recommend buying medicine before you start this mission.
Go hang from the back side of the cliff and drop down.
Run straight ahead, don't mind the guard on the tower.
Go left at the end of the end of the lane and keep running staright ahead.
Wait until this guard has his back towards you.
Climb the building.
Run towards the end of the building and walk over the iron ropes.
Jump from the building into the haystack.
Wait until this guard hashis back turned to you and sneak in behind him.
Walk to the blueprints and set them on fire.
Climb the boxes.
Jump towards the roof and grab on.
Climb up the roof and walk towards the end.
Hang from the ledge and drop down.
Ignite the barrels.
Run away from the barrels and into the mark.
After the cutscene, you'll find yourself on a horse. Just follow the carriage until the next cutscene.
The carriage will ride itself, all you need to do is steer.
When guards ride beside you, ride into them to kill them.
Just keep aiming and shooting at the guards until the mission is over. When you're low on life you can take the medicine to heal.
The start of the final mission of this sequence is at your hide out.
After the cutscene, walk up to the map and interact with it.
When you have left the menu, run towards the door and leave the building. This will end the sequence.
Sequence 5: part 1
Walk up to the brothel and talk to claudia to start this mission.
Run towards the mark on your map. After the cutscene, kill the guards that are attacking Egidio.
Walk towards the marks on your map, make sure you stay away from the guards.
When this mission is over, you can start the next one immediately. You will need 3000 florentines to start.
Go to the mark on top of the roof.
Jump from roof to roof tailing the guards and stealth assassinating the guards.
Once you've reached this point, just wait for the cutscene.
When the cutscene is over, jump down and run towards the back of the pantheon. Climb the broken column.
From the column, jump on the platform shown in the picture.
From the platform, climb up the panthon and run towards the mark.
After the cutscene, go hang from the ledge and climb down.
Keep climbing down until you've reached the ledge.
Run along the edge and air assassinate the guard.
Walk straigh forward into the first street.
Take the first street to your left.
Walk trough the street and take a right.
Go left at the split.
Go left again.
Go left down the stairs.
Run across the market and past the right side of the church.
Go right up the stairs.
At the top of the stairs, go left.
Go right at the end of the hallway.
Keep following the street until you've reached the stairs at your left.
Run over the bridge until another cutscene starts to play.
Tail the guards, either by hiring the courtesans or just blending in with groups of people.
When you've reached this point, wait until the box has been passed on, then run up the stairs
Keep tailing the guards.
After the cutscene, hire the courtesans to follow you so you can blend in with them. Then go towards the mark.
If you've lost the courtesans, you can always kill guards using your recruits.
When you've reached this point, run towards the wall shown in the picture and climb it.
Drop down on the other side and another cutscene will commence.
After the cutscene, jump into the haystack and kill the guard.
Run up the wall show in the picture and climb over it.
Select your poison blade.
Run towards the guard and poison him.
Go sit on a bench and wait for the guard to kill 'The Banker'. After that just jump over the ledge into a haystack and the mission will be over.
For the last mission, just run in the marker and after the cutscene, this sequence will be over.
Sequence 6: part 1
After the cutscene, run towards a handle with as few guards as possible.
Kill all the guards around it.
When there are no guards around, try to close the gate. you will be attacaked but closing the gate only takes 2 seconds so it shouldn't be to hard.
When the cutscene is over, just get on a horse and follow Bartolomeo.
You can start the next mission immediately. NOTE: for 100% synchronization you will need the poison darts from Leonardo Da Vinci.
I started with the closest camp (shown in the picture).
Begin with killing the first pair of guards with your hidden blade. (NOTE: this is easier with the double hidden blade you can buy from Leonardo Da Vinci).
Locate the second pair of guards and sneak up on them. Make sure the guards inside of the camp can't see you.
Run to the side and poison one of the brutes with the darts.
While all the attention will be pointed at the brute, enter the camp and either sneak up on the guards (not reccomended since they might see you), kill the with the crossbow or poison some more and let them kill each other.
The second camp i took was the one closest to the first one.
Begin with killing the guards on the look-out towers with your crossbow.
Then climb the tower at the outside of the camp and poison one of the brutes.
Jump down and poison another guard. You then can easily kill the other ones.
Sequence 6: part 2
Follow Bartolomeo and his mercenaries.
Four guards will attack, just kill them and keep following the group.
When you're at the road block, you'll need to kill 3 guards. Begin with the one on the roof.
Run to the other roof and air assassinate the second guard.
Then just walk up to the third one and kill him.
For the second roadblock, begin with killing the one that is patrolling on the left.
Then just wak up to the one at the back and kill him.
You can now kill the third one anyway you like.
For the last 3, begin with jumping in the cart with hay and killing the first guard.
Then climb the back of the building and kill the second guard.
You can air assassinate the third guard from the roof.
Now just keep followoing the mercenaries inside the french camp.
After the cutscene, kill the attacking guards.
When the guards are dead, climb the one of the roofs and run towards the tower.
Kill the guards on the tower.
Run towards the other towers and kill the guards on them too.
Jump down and kill the four guards defending the gate.
Run towards the handle and open the gate.
Run trough the gate and go to the right. Kill the guard before he detects you.
Run up the building and kill the guard.
Climb on the roof and highlight your target.
press the 'attack' button to air assassinate him.
Sequence 7: part 1
Move towards the beam with the knifes and interact with it to start this mission.
Once outside you will be attacked. kill all the attackers.
Get on a horse and follow La Volpe.
When you get to the thieve, get off your horse and kill the guards.
Get on your horse and follow La Volpe again.
When you reach the second thief, just get off your horse again and kill the guards.
Get back on your horse and follow La Volpe once more.
When he stops, locate the last thief with eagle vision.. He will be at the other side.
Jump towards him and kill all of the attacking guards.
Sequence 7: part 2
Start by getting on a horse and following micheletto. Make sure you don't come too close to him or he will detect you.
When he stops, run to the side and climb up the wall.
Climb up the structures and run towards him.
When the cutscene is over, highlight your target and call your recruits to kill him.
You wont have a horse this time but you can easily follow him on foot.
Run to the side and enter trough on of the unguarded gates.
Get on the walls and follow him.
When you see a guard, you can kill them with your crossbow.
After the cutscene, make sure one of your targets is highlighted and call your assassins to kill them.
Jump down and follow him. You can take a horse, but once again this is easily doable on foot.
When he stops again, climb up the wall on the left side of the guards.
Run trough the window and a cutscene will commence. When the cutscene is over, immediately jump forward into the other window so he wont see you. From there, highlight the guards and let your recruits kill him. Then follow him again.
Once he reaches the colloseum, go towards the mark and start climbing it.
When you are at the top, a cutscene will commence and you'll have 5 minutes to kill 4 guards. the easiest way to do this is by hanging from the ledge as shown in the picture.
Get onto the broken parts of the wall shown on the screen and run along them.
Wehn you've reached the first guard, you can easily air assassinate him. The other 3 can easily be illed either with a crossbow or by sneaking up on them.
Jump down and into the mark. After the cutscene, simply walk towards the mark on your map.
Walk towards the middle of the patch of hay on the ground.
When you can move again, walk towards the patch of hay at the other side.
When you can walk again, walk onto the middle of the last patch of hay.
When the cutscene is over, use eagle vision to locate your target.
Walk towards him and kill him.
After the cutscene, carry the man outside and towards the doctor. Make sure the guards have passed or they will get suspicious.
After yet another cutscene, start sprinting behind the running man.
Any guards can be easily dodged.
When you are close enough, tackle the man.
When the cutscene is over, start sprinting towards the mark.
For 100% synchronization you can't swim, so use the bridge that you can see in the background of the picture.
For the final mission of this sequence, just enter the door of your hideout and let the cutscene play.
Sequence 8: part 1
Walk trough the mark to start this mission
Wait until the guard's back is turned at you, then run towards him and kill him.
Climb the ladder and climb ontop of the wall.
Make sure the guard had his back turned towards you so you can kill him.
Kill the other guard with either your crossbow or by sneaking up on him.
Climb the tower and walk into the mark.
Run right along the wall and kill the guard that's on the lookout.
Killing this guard is optional.
Climb the roof and kill the guard.
Jump from beam to beam until you get to the other side.
Kill the guard either with your crossbow, or by running on the wall and pulling him down.
Jump down and climb the stairs. When you're at the top, go towards the door and interact with it.
After the cutscene, run up the wall and grab hold of the ledge.
Move along the wall and sling from the iron pole towards the wooden beam.
From the beam, run up the wall and grab the ledge.
Move along the edge around the corner and drop down onto the wooden beam.
Jump from beam to beam until you reach the one in the picture.
Hang from this beam and move along the ledge.
Keep moving along the ledge until you've reached the iron pole. Swing towards the wooden platform.
From the platform, climb up. Watch out for the patrolling guard. Warning: there is another one around the corner.
Run up the stairs until you've reached the library.
Kill the guard that's walking around the room.
Climb the bookcase and go hang from the balcony.
Make sure the guard can't see you when you climb up. Kill him with your hidden blade.
Run towards the window, watch out for the guard.
Go hang from the window at the outside.
Move along the wall until you've reached the mark.
Go hang from the wall at the outskirts of the building and move along it until you've passed the guards.
Climb up the roof and jump towards the window.
After the cutscene, run down the stairs.
Keep running down and into the mark.
After Cesare closed the door, run back up the stairs and run up the wall. From there jump back onto the platform.
Jump from beam to beam and run up the wall.
Climb up the wall and just run past the guard.
Sprint up the stairs, ignore the guard.
Jump down from the tower into the haystack.
Jump out of the haystack and run towards the mark.
Jump over the wall and into the haystack.
Run towards the mark and trough the big doors. Ignore everyone that attacks you.
Run towards the mark, you can just wait there until you're anonymous.
Sequence 8: part 2
After the cutscene, guards will attack you. just press your attack button when they're close enough and run away.
Run out of the Vatican.
When you've left the Vatican, swim or run away from the guards until you're anonymous.
After the cutscene, you will be needed to kill 5 guards. Just go stand close to them and hold your attack button until your life is at 0.
After yet another cutscene, go towards the highlighted area. Run around until you see the Cardinal.
Tail the cardinal.
You can kill the guards with your apple.
Wait for him to walk into the center of the theater, another cutscene will begin.
Make sure you're close to the two guards and hold your attack button.
When the guards are dead, run around until your life is restored. Repeat this stap twice more to finish this mission.
Sequence 8:part 3
Run towards the gate with Cesaro and his guards.
Kill the attacking guards with your apple.
You can also just go hide ontop of the roofs until the two minutes have passed.
When the timer is over, jump down and go stand among your brothers.
After a very short fight with the guards, get on your horse and ride towards the mark.
When your horse dies, continue on foot.
When you've reached the mark, kill the attacking guards.
Now repeat the previous step.
Climb the tower and go inside of it. Keep going up via the ladders and wait for the cutscene.
Run towards the last mark and kill the attacking guards.
Start climbing the second tower and climb up the wall.
Run into the mark and let the cutscene play.
To kill Cesare, break his defence and start hitting him repeatedly. Repeat this step until he's dead.
When the guards attack, kill them using chain kills.
After the cutscene, you will be controlling Desmond. Run up the stairs and activate eagle vision.
When you're at the theater, the parkour you'll need to do should be pretty self-explanatory.
Once you're inside, activate eagle vision and interact with the pillars.
Keep following the parkour path.
Once again, activate eagle vision and interact with the pillars.
When you're in the church, run up the shrine, climb the cross and jump towards the handle.
Activate the handle.
Jump from beam to beam until you reach the platform.
Activate the handle on the platform.
Jump down and go to the activation handle.
Run towards the big wall and wait for the cutscene.
Jump down and activate the handle.
Parkour to the handles and activate them.
When the 'walls' go back in the ground, you've activated the wrong handle. Climb back and activate the one at the other side of the pillar.
When all the handles are activated, run towards the apple.
After the cutscene, move forward.
When you're close enough, press any button.
After the end credits you will regain control over Ezio. You can get back to rome by leaving trough the door at your right.
You are now free to do whatever you want. I hope this walktrough was helpful and have fun playing!